Use our CleanCap® Cyanine-5 labeled mRNA to visualize and analyze mRNA delivery into and localization in cells using fluorescence microscopy.
We offer dye-labeled enhanced green fluorescent protein (EGFP) and Firefly luciferase (FLuc) mRNAs, both transcribed with Cyanine 5-UTP:5-Methoxy-UTP at a ratio of 1:3. Cyanine 5 has an excitation maximum of 650 nm and an emission maximum of 670 nm. Substitution in this ratio results in mRNA that is easily visualized and that can still be translated in cell culture. Substitution with Cyanine 5, however, reduces translation of EGFP and Fluc.